AUTHOR(S): 

Ghadeer A. R. Y. Suaifan, Mohammed Zourob

TITLE

Paper Based Biosensor for Protease Detection Using Magnetic Nanoparticles

KEYWORDS

Protease, Prostate Cancer, Prostate Specific Antigen, Colorimetric, Biosenser, Magnetic nano-particles

ABSTRACT

Prostate cancer is the leading cause of cancer-related deaths in male, aside from lung cancer. Prostate cancer is commonly treated by radical prostatectomy. However, fifteen years following prostactomy, recurrence was possible in approximately 40% of patients. Proteolytically-active prostate specific antigen (PSA) level perturbation was found to be useful tool for detecting biochemical relapse after radical prostatectomy. Current diagnostic methods do not discriminate between proteotically-active and inactive PSA forms. Thus, a paper-based biosensor for the evaluation of active PSA level was constructed. The biosensing configuration is based on the detection of PSA amylodic activity using a specific peptide substrate, sandwiched between magnetic nanonanoparticles and gold sensing platform placed on the top of a paper support. Upon PSA introduction, an external magnet fixed at the back of the sensor sopport would accelerate the cleavage of the magnetic nanoparticles-peptide moieties away from the surface. The colour change resulting from this dissociation was detected by the naked eye. This biosensor was amenable for a qualitative and quantitative PSA detection in buffer and in spiked urine samples with a lower detection limit of 1 ng/mL and 5 ng/mL, respectively. In conclusion, the designed colorimetric biosensor pinpointed superiority in criteria's including sensitivity; simplicity and fast processing time.

Cite this paper

Ghadeer A. R. Y. Suaifan, Mohammed Zourob. (2017) Paper Based Biosensor for Protease Detection Using Magnetic Nanoparticles. International Journal of Oncology and Cancer Therapy, 2, 1-3