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AUTHOR(S): 

Ayse Ak Can, Gamze Bolukbasi Ates, Hakan Solmaz, Ozgur Kaya, Murat Gulsoy

 

TITLE

Laser Light Induced Cytotoxicity on Normal and Cancer Cell Lines

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KEYWORDS

635 nm, 1071 nm, Laser, Cell viability, Glioblastoma, Fibroblast

ABSTRACT

Laser lights with photosensitizers can be used to destroy various cancer in photodynamic therapy. However, solely laser irradiation can also cause photochemical interactions resulting in cytotoxicity depending on the wavelength, energy dose and the cell source. Fibroblast and glioblastoma cells were irradiated with laser light at 635 nm and 1071 nm to deliver energy doses of 15, 30, and 60 J/cm2. MTT was used to determine viability of cell lines. Statistical analysis was performed using ANOVA. The viability of glioblastoma was decreased significantly by laser exposure at both wavelengths. The viability of fibroblast was not affected by laser irradiation with the same parameters. While lasers were applied, temperature rise was not observed. In photodynamic therapy, photochemical or photothermal effects occur if laser is combined with a photosensitizer. On the other hand, our preliminary results show that when only laser, without a photosensitizer, is used, it has cytotoxic effect on cancer cells, not on normal cell, especially at lower energy densities of 635 nm and 1071 nm. These data contain valuable outcomes especially for photodynamic therapy which is used to treat cancer patients. Effect of laser exposure has to be taken into consideration when conducting PDT, depending on cell type.

Cite this paper

Ayse Ak Can, Gamze Bolukbasi Ates, Hakan Solmaz, Ozgur Kaya, Murat Gulsoy. (2016) Laser Light Induced Cytotoxicity on Normal and Cancer Cell Lines. International Journal of Oncology and Cancer Therapy, 1, 7-11