Evaluation Semen Characterization of Roosters Resulted from Different Local Lines and Their Crosses with ISA Brown

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Poultry Genetic Groups, Age, Semen Parameters, Wattles Measurements.


This study conducted at Gardarash station, College of Agriculture in collaboration Directorate of Agricultural Research–Erbil during June and July, 2014. A total of 92 roosters were belongs to three local lines and their main and reciprocal crosses with ISA brown. The semen collected with abdominal massage technique into tube maintained at 38-39oC. Volume, pH and colour score of the semen were recorded. The wave patterns, individual motility, percentages of live/dead and abnormal/normal sperms were evaluated by using Eosin/Nigrosine stains under light microscope. Length and width of wattles were measured using Digital Caliper while the colour of wattles score recorded visually. SAS program used to study the effect of genetic groups and age of roosters on studied traits. The above traits were reanalyzed to study their affects by colour of semen and by colour of wattles. Duncan Multiple Range Test conducted to diagnosing the significance differences between the means of the levels of each factor. Correlation coefficients among the studied traits were calculated. Overall mean of ejaculate volume, sperm concentration, live sperms, abnormal sperms, mass motility, individual motility and pH value were 0.369 (ml), 4.47 (n*109)/ml, 91.35%, 12.01%, 68.40%, 75.60% and 7.12 respectively. There were significant differences among genetic groups in ejaculated volume, sperm concentration, abnormal sperm%, mass motility% and individual motility% only. Effect of age of roosters was significant on mass and individual motility only. Higher negative and significant correlation was between abnormal sperm with live sperm (-0.49), while the higher positive and significant correlation was between mass motility and individual motility (0.95). Overall means of wattle length and width were 55.61 and 46.46 (mm) respectively. The genetic group (local black with brown neck) excelled the other pure and crossed genetic groups significantly in their wattle length and width. Effect of age of roosters found to be not significant on both traits. The correlation between length and width of the wattles was highly significant (0.79). Roosters with creamy semen was significantly better than roosters with watery semen in ejaculate volume, sperm concentration, abnormal sperms and mass motility, while the roosters with milky semen had moderate values. The differences in all semen parameters according to the colour of wattles were not significant. Roosters having brown wattles had significantly greater length and width comparing with those having redden white wattles. Conclusion: The semen parameters must be evaluated individually. The results obtained from this study will help to do some further researches on freezing of semen roosters and artificial insemination.

Cite this paper

Hani N. Hermiz, Ahmed S. Shaker, Basheer M.A. Hasafa, Tahir R. Al-Khatib, Sardar Y. Sardary, Jamil S. Toma. (2016) Evaluation Semen Characterization of Roosters Resulted from Different Local Lines and Their Crosses with ISA Brown. International Journal of Agricultural Science, 1, 7-14