Kazuki Hiro, Momoko Imanishi, Ayuko Itsuki, Shigeto Nakamura, Hirotsugu Shimada, Hiroshi Fukuoka
Negative pressure of liquids is expected to inactivate bacteria by the pressure which is much lower in magnitude than positive pressure. Nevertheless, the pressure is difficult to be generated experimentally due to cavitation through heterogeneous nucleation. In order to check the expectation, a metal Berthelot tube was newly developed, solutions including bacillus subtilis experienced negative pressure repeatedly, and a number of colonies was counted by an agar dilute plate method. Results indicated that colonies which experienced negative pressures were less than those for non-treatment, and reduction ratios increased with numbers of repetition. The application of the Berthelot method will lead to a new means without any chemical compounds in future.
Negative pressure of liquid, Berthelot method, Cavitation, Inactivation of bacteria
Cite this paper
Kazuki Hiro, Momoko Imanishi, Ayuko Itsuki, Shigeto Nakamura, Hirotsugu Shimada, Hiroshi Fukuoka. (2022) An Application of a Berthelot Method Using a Metal Tube to Inactivation of Bacillus Subtilis. International Journal of Biology and Biomedicine, 7, 30-33